جداسازی و شناسایی مخمرRhodotrula sp. به ‌عنوان سویه‌ای بومی توانمند در تولید بیوسورفکتانت از خاک های آلوده نفتی

نوع مقاله: علمی ترویجی

نویسندگان

1 کارشناس ارشد زیست فناوری مکروبی

2 عضو هیئت علمی دانشگاه تهران

چکیده

بیوسورفکتانت‌ها ترکیبات فعال سطحی هستند که توسط بسیاری از میکروارگانیسم‌ها تولید می‌شوند. این ترکیبات دارای کاربردهای بسیاری در صنایع گوناگون به ‌ویژه در صنعت نفت و پاک ‌سازی آلودگی‌های نفتی هستند. در این پژوهش از میان 22 جدایه‌ی مخمر جداسازی شده از 8 نمونه خاک آلوده به هیدروکربن‌های نفتی در نهایت جدایه‌ی G-13 با قطر هاله‌ی 1/13 سانتی ‌متر و کاهش کشش سطحی به میزان 26 میلی ‌نیوتون بر متر به ‌عنوان جدایه‌ی برتر انتخاب گردید. نتایج شناسایی مشخص کرد که این جدایه شباهت 100% با Rhodotrula sp. داشته و با کد UTMC 5032 در کلکسیون میکروارگانیسم‌های دانشگاه تهران ثبت گردید. شاخص امولسیون ‌زایی (EI1 و EI24) این جدایه برای تولوئن و دیزل برابر 100% تعیین شد. بر نتایج سویه‌ی Rhodotrula sp. توانایی بالایی در تولید ترکیبات زیستی فعال سطحی و ایجاد امولسیون با ترکیبات آ‌ب ‌گریز دارد و می‌تواند به‌عنوان سویه‌ای توانمند در حذف آلودگی‌های نفتی معرفی شود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Isolation and characterization of an indigenous biosurfactant-producing Rhodotrula sp. from oil contaminated soil

نویسندگان [English]

  • Parvin Hasani Zadeh 1
  • Hamid Moghimi 2
2 Assistant professor in University of Tehran
چکیده [English]

Biosurfactants are surface-active agents produced by most microorganisms. These compounds have various applications in several industries especially in the oil industry and bioremediation of oil contaminations. In this study among 22 isolated yeast strains, G-13 was selected as the best biosurfactant producing isolate by reducing the surface tension to 26 mN/m and creating a clear zone with 13.1 cm diameter. Molecular identification revealed that G-13 had 100% similarity to Rhodotrula sp. and deposited by accession number UTMC 5046 in the University of Tehran Microorganism Collection. Emulsification index (EI1 and EI24) of the Rhodotrula sp. UTMC 5046 was determined 100% for toluene and diesel. According to the results, Rhodotrula sp. has a high potential in biosurfactant production and emulsification of hydrophobic compounds so it can be used in bioremediation of oil contamination.

کلیدواژه‌ها [English]

  • Biosurfactant
  • Bioremediation
  • Surface tension
  • Yeast

مراجع

  1. Rahman P.K. and Randhawa K.K.S., Editorial: Microbiotechnology based surfactants and their applications, Frontiers in microbiology, Vol.6, 2015.
  2. Sekhon K.K., Khanna S. and Cameotra S.S., Biosurfactant production and potential correlation with esterase activity, Journal of Petroleum & Environmental Biotechnology, Vol.3, 2012, pp 1-10.
  3. Banat I.M., Biosurfactants production and possible uses in microbial enhanced oil recovery and oil pollution remediation: a review, Bioresource technology, Vol.51, 1995, pp 1-12.
  4. Fakruddin M., Biosurfactant: production and application, Journal of Petroleum & Environmental Biotechnology, Vol.3, 2012, pp 1-5.
  5. Hommel R.K., Formation and function of biosurfactants for degradation of water-insoluble substrates, in Biochemistry of microbial degradation, 1994, Springer, pp 63-87.
  6. Amaral P.F., Coelho M.A.Z., Marrucho I.M. and Coutinho J.A., Biosurfactants from yeasts: characteristics, production and application, in Biosurfactants, 2010, Springer, pp 236-249.
  7. Bhardwaj G., Cameotra S.S., and Chopra H.K., Biosurfactants from fungi: A Review, Journal of Petroleum & Environmental Biotechnology, Vol.4, 2013, pp 160-166.
  8. Ekundayo F.O., Olukunle O.F. and Ekundayo E.A., Biodegradation of Bonny light crude oil by locally isolated fungi from oil contaminated soils in Akure, Ondo state, Malaysian Journal of Microbiology, Vol.8, 2012, pp 42-46.
  9. Willumsen P.A. and Karlson U., Screening of bacteria isolated from PAH-contaminated soils for production of biosurfactants and bioemulsifiers, Biodegradation, Vol.7, 1996, pp 415-423.
  10. Konishi M., Morita T., Fukuoka T., Imura T., Kakugawa K. and Kitamoto D., Production of different types of mannosylerythritol lipids as biosurfactants by the newly isolated yeast strains belonging to the genus Pseudozyma, Applied microbiology and biotechnology, Vol.75, 2007, pp 521-531.
  11. Qazi M.A., Kanwal T., Jadoon M., Ahmed S. and Fatima N., Isolation and characterization of a biosurfactant‐producing Fusarium sp. BS‐8 from oil contaminated soil, Biotechnology progress, Vol.3, 2014, pp 1065-1075.
  12. Techaoei S., Leelapornpisid P., Santiarwarn D. and Lumyong S., Preliminary screening of biosurfactant producing microorganisms isolated from hot spring and garages in northern Thailand, KMITL Science and Technology Journal, Vol.7, 2007, pp 38-43.
  1. Kuiper I., Lagendijk E.L., Pickford R., Derrick J.P., Lamers G.E., Thomas‐Oates J.E., Lugtenberg B.J. and Bloemberg G.V., Characterization of two Pseudomonas putida lipopeptide biosurfactants, putisolvin I and II, which inhibit biofilm formation and break down existing biofilms, Molecular microbiology, Vol.51, 2004, pp 97-113.
  1. Cooper D.G. and Goldenberg B.G., Surface-active agents from two Bacillus species, Applied and environmental microbiology, Vol.53, 1987, pp 224-229.
  2. Green M.R. and Sambrook J., Molecular cloning: a laboratory manual, Vol.1, 2012: Cold Spring Harbor Laboratory Press New York.
  3. Walter V., Syldatk C. and Hausmann R., Screening concepts for the isolation of biosurfactant producing microorganisms, in Biosurfactants, 2010, Springer, pp 1-13.
  4. Banat I.M., Franzetti A., Gandolfi I., Bestetti G., Martinotti M.G., Fracchia L., Smyth T.J. and Marchant R., Microbial biosurfactants production, applications and future potential, Applied Microbiology and Biotechnology, Vol.87, 2010, pp 427-444.
  5. Bustamante M., Durán N. and Diez M., Biosurfactants are useful tools for the bioremediation of contaminated soil: a review, Journal of soil science and plant nutrition, Vol.12, 2012, pp 667-687.
  6. Guerra-Santos L.H., Käppeli O. and Fiechter A., Dependence of Pseudomonas aeruginosa continous culture biosurfactant production on nutritional and environmental factors, Applied Microbiology and Biotechnology, Vol.24, 1986, pp 443-448.
  7. Das K. and Mukherjee A.K., Characterization of biochemical properties and biological activities of biosurfactants produced by Pseudomonas aeruginosa mucoid and non-mucoid strains isolated from hydrocarbon-contaminated soil samples, Applied microbiology and biotechnology, Vol.69, 2005, pp 192-199.
  8. Bodour A.A., Drees K.P. and Maier R.M., Distribution of biosurfactant-producing bacteria in undisturbed and contaminated arid southwestern soils, Applied and Environmental Microbiology, Vol.69, 2003, pp 3280-3287.
  9. Youssef N.H., Duncan K.E., Nagle D.P., Savage K.N., Knapp R.M. and McInerney M.J., Comparison of methods to detect biosurfactant production by diverse microorganisms, Journal of Microbiological Methods, Vol.56, 2004, pp 339-347.
  10. Andrade Silva N.R., Luna M.A., Santiago A.L., Franco L.O., Silva G.K., de Souza P.M., Okada K., Albuquerque C.D., Silva C.A. and Campos-Takaki G.M., Biosurfactant-and-bioemulsifier produced by a promising Cunninghamella echinulata isolated from Caatinga soil in the northeast of Brazil, International journal of molecular sciences, Vol.15, 2014, pp 15377-15395.
  11. Yin H., Qiang J., Jia Y., Ye J., Peng H., Qin H., Zhang N. and He B., Characteristics of biosurfactant produced by Pseudomonas aeruginosa S6 isolated from oil-containing wastewater, Process Biochemistry, Vol.44, 2009, pp 302-308.
  12. Bento F.M., de Oliveira Camargo F.A., Okeke B.C. and Frankenberger W.T., Diversity of biosurfactant producing microorganisms isolated from soils contaminated with diesel oil, Microbiological research, Vol.160, 2005, pp 249-255.
  13. Amani H., Sarrafzadeh M.H., Haghighi M. and Mehrnia M.R., Comparative study of biosurfactant producing bacteria in MEOR applications, Journal of Petroleum Science and Engineering, Vol.75, 2010, pp 209-214.
فصلنامه علمی ترویجی فرآیند نو

دوره 12، شماره 59
پاییز 1396
صفحه 92-105

فایل ها

اشتراک گذاری

ارجاع به این مقاله

آمار