In the upcoming study, the 175 kilometers oil products pipeline is simulated by Pipeline Studio software, the equipment needed to remove fluid from the pipe has been installed at different points of the pipeline, and a number of ten removal tests have been performed. In each test, the operational data related to the pipeline and other data are recorded and the results of each stage of the tests are compared with the outputs of the software model. In the following, two methods to identify the location of the leak in the pipeline including negative pressure waves and the intersection of the hydraulic gradient have been introduced and they have been used to calculate the location of the leak in the tests. The calculation results showed that both methods had acceptable accuracy in finding the leak location, but the comparison of the two methods showed that negative pressure waves had a better performance (11% error vs. 18%) in estimating the leak location. The high correspondence between the diagrams extracted from the software and the real conditions ensures that the Pipeline Studio software can be used not only in the pipeline design phase but also in the analysis of operational changes and its results could be help in a better understanding of pipeline.Keywords: leak detection in pipeline, negative pressure waves, hydraulic gradient line, oil transmission pipeline, pipeline simulate with Pipeline Studio.